The Efficacy Of Ck2 Inhibitors Essay - 974 Words

AML xenograft model: For the proposed studies to test the efficacy of CK2 inhibitor in AML, we have transduced the human AML cell line, U937 and human APML cell line, HL60 with a lentiviral vector carrying the luciferase gene. The luciferase-producing U937 and HL60 cells were injected via the tail-vein into NOD.Cg-Rag1 Il2rg/SzJ (NRG) mice. After 3-5 days, engraftment of AML cells was confirmed by bioluminescence imaging documenting one log increase in quantitative signal measured by the IVIS Xenogen reader. Pilot results in two animals demonstrate that both U937 and HL60 luciferase-producing cells successfully established leukemia (Fig 6). Thus, we have demonstrated the feasibility of the proposed experiments by establishing two luciferase tagged AML murine xenografts models in which to assess therapeutic efficacy of CX-4945. Experimental approach: 2.1) Test the therapeutic efficacy of CX-4945 in AML cell line derived murine xenograft model We will use 4-6 weeks old female, NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl/SzJ (NRG) mice to create AML xenograft models. Ten mice per treatment group will be injected with 2x106 luciferase expressing U937 cells via tail vein. After confirming engraftment by using bioluminescence imaging, treatment with CX4945 will be initiated. Visible signal or one log increase in signal will be used as indication of engraftment. The treatment group will receive CX4945 at 100mg/ kg via oral gavage, five days a week for three weeks and the control group will

Reality TV Essay - 1074 Words

Reality TV: The Rise of a New Show â€Å"The Contender† nbsp;nbsp;nbsp;nbsp;nbsp;One of the newest shows to hit the vast majority of reality TV shows that already exist is The Contender. The Contender is a show that focuses on all the hardships, training, pain, and dilemmas that a boxer, who is preparing to fight, must endure. The Contender will be a very successful show, because it has the attributes of raw emotion that can’t be found when a director is yelling ‘action,’ and ‘cut.’ By raw emotion I mean that the viewer has reason to watch the television show due to an emotional attachment, besides the mere entertainment value, or for the love of the sport of boxing. Viewers fall in love, but instead of characters, they are able to fall†¦show more content†¦The Contender is centered on a group of professional boxers who are contending for a one million dollar prize. They essentially must fight one another in single elimination five round fights. The fights are set up and the fighters are chosen by one of the two teams that win the challenge before the fight. The rules of the game are established, but this is the backdrop to what the show truly is. The show is a success due to the battle of emotion, not the battle of fists between the men in the ring. The rules for reality television shows, are just measures to evoke emotion. Other great examples are the betrayals and voting in Survivor, or the decisions in The Bachelor. The Contender accomplishes what all of the great reality television shows accomplish. The raw emotion is brought to the viewer through a family member, loved one, or even a mentor. The Contender establishes a background story for every boxer allowing for a connection to be made between the viewer, and the contestant. The connection is what allows for the viewer to continue watching for the weeks ahead. These men are shown training for their goal, and shown putting in the extra work to make a dream come true. However, one thing that The Contender has that is not found in many other shows, and a reason for its success, is the determination and drive of the competitors. The twist to The Contender is that all of these men have beenShow MoreRelatedThe Reality Of Reality Tv908 Words   |  4 PagesToday’s audience grabs hold of reality TV now a day. Looking forward week to week to watch these unscripted real life situation shows. In a way it s becoming increasingly hard to avoid not watching. Some viewers see the TV show and tend to be attention seekers, and reality TV allows them to fantasize about achieving status through instant fame. Too much reality TV may lead viewers to idealize real world situations, like romanticizing dating. Like when Truman saw the girl in the library who was anRead MoreThe Reality Of Reality Tv991 Words   |  4 PagesI truly believe that reality TV needs a different name. 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According to Jennifer Pozner’s â€Å"Ghetto Bitches, China Dolls, Cha Cha Divas† reality shows contradict themselves. Pozner points out that a contestant was asked what sheRead MoreReality Tv Shows855 Words   |  4 PagesBased on these criteria qualified industries were call centres and reality TV shows. In deciding between these alternatives, I interviewed a few stakeholders within the call centre and media industries. The feedback was that there is minimal use of video interviewing within these industries, which suggests a blue ocean opportunity. In addition, we had a conversation with the CEO of Kira Talent. We discovered that Kira Talent had pitched to comp anies with huge call centre operations previously, butRead MoreThe Race On Reality Tv866 Words   |  4 PagesRace on Reality TV A unique feature of reality TV makes the show as a site where we can find how the tolerance is conducted in real life, in terms of race, gender, and foreignness. As an â€Å"unabashedly commercial genre united less by aesthetic rules or certainties than by the fusion of popular entertainment with a self-conscious claim to the discourse of the real’’ (Murray Ouellette, 2004, p. 2), reality TV enjoys its privilege of dealing with the controversial issue such as racism with ease, through

Is Genetic Testing A Type Of Medical Test - 2264 Words

To screen, or not to screen, is the question. Genetic testing is a type of medical test that identifies changes in chromosomes, genes, or proteins. The results of a genetic test can confirm or rule out a suspected genetic condition, help determine a person’s chance of developing or passing on a genetic disorder or link up and reunite lost family lineage, ties (Sofge, 2014). For instance, the American reality TV star Snooki once thought she was Italian-American but her skin color was too dark. A genetic test showed that she had been adopted and her parents came from Chile instead. Regardless of the myriad benefits of genetic testing in solving various human mysteries and identification of people in criminal cases (innocent or guilty),†¦show more content†¦A chromosomal genetic test technique analyzes the whole chromosomes or long lengths of DNA to verify if there are any large genetic changes, such as an extra copy of a chromosome that cause a genetic condition of conc ern to one’s health ( Scuffham MacMillan, 2014). The biochemical genetic tests technique study’s the amount or activity levels of proteins; abnormalities in either may indicate changes to the DNA that result in a genetic disorder. Evidently, genetic testing can provide information about a person’s genes and chromosomes. The available types of genetic testing include: The newborn screening that is performed after birth to identify genetic disorders that can be treated early in life. In the United States (U.S), millions of expecting parents provide consent for their babies to be tested each year. Currently, almost all states test infants for phenylketonuria (PKU), a genetic disorder that causes intellectual disability and congenital hypothyroidism, a disorder of the thyroid gland if left untreated. Diagnostic type of genetic testing is done before or after birth to identify or rule out a specific genetic, chromosomal condition. In many cases, it is used to confir m a diagnosis in suspicious condition based on physical signs and symptoms (Scuffham MacMillan, 2014). The results of a diagnostic

It Is the Minor Characters Who Create Much of the Interest in Jane Eyre free essay sample

Minor Characters often act as the catalysts for larger events with in a characters life; it is through interaction with people around them that our protagonists are defined and redefined. It is thought this interaction and the interest individual characters evoke that make minor characters with in both these novels so vital. This essay will explore how minor characters with in the novels ‘Jane Eyre’ and ‘Wide Sargasso Sea’ impact upon the lives of our central characters; as well as the interest they bring through their individual complexities, and how this in turn creates much of the interest with in these novels. Within the troubled childhoods of our protagonists particular importance is often placed upon the parental figures with in there early lives. Within ‘Jane Eyre’ Mrs Reed and Mr Brocklehurst are the most notable examples of this. Mrs. Reed and Mr. Brocklehurst both take the needy in to their care, but do not take care of them. Mrs. Reed abuses Jane and allows her children, especially her son, John to abuse her behind her back. Mr. Brocklehurst is far worse and attempts to justify himself through the farce of education, justifying his cruelty by claiming that his ‘aim is not to pamper the body but strengthen the soul’. Whilst both appear at first glance to be generous benefactors, they are in fact disguising a rotten core- this acts as an interesting metaphor for the corruption within the class system- and is a continuing theme throughout the novel, as we later notice the wealthy exterior of Thornfield disguises a dark secret. Similarly Annette also experiences injustice with in her childhood from people in positions of guardianship and authority; the most notable example being Annette, her mother. It is Annette’s cruelty and her mysterious death that creates much of the interest with in the novel. An example of this indifference is when Annette wishes to leave Jamaica and comments ‘It is not safe for Pierre’, in this moment it is as if Annette forgets she has another child, creating sympathy for Antoinette from the audience and causing us to wonder, why? Just like Jane’s early disconnection with her parental/custodian figures Annette’s lack of bond with her mother shapes the character she will later become, and engages the reader by providing a character who has many depths and who has the ability to be altered throughout the novel. Annette’s death also presents another point of interest for the reader, Antoinette comments upon her passing casually and we are never made aware of how she truly died. This foreshadows the mysterious death of Antoinette and as the reader is already aware at this point of Annette’s mental deterioration we begin to wonder how it is that the novel will end. Interestingly this proceeds in to a cycle with in Jane Eyre as Bertha’s death causes the reader to question: what will become of Jane? Helen Burns acts as a very positive point of interest with in Jane Eyre. It is firstly important to note the name Bronte used for ‘Helen Burns’. Firstly fire has positive connotations in ‘Jane Eyre’ and is often associated with positive destruction, as the name Helen can be translated to ‘reed’ this may refer to the destruction of Mrs Reed’s negative impact upon Jane. It also seems to have religious connotations as it sounds like a Minimal Pair of Hell burns, which mirrors Helen’s deeply religious character. The biggest impact Helen has with in the novel is that she teaches Jane the lesson of forgiveness; whilst Jane begins her time at Lowood believing that’s she should ‘fight fire with fire’ Helen teaches her to ‘return good for evil’. This is significant as later in the novel Jane proves this lesson learnt when she forgives Mrs Reed at her death bed. Helens influence may also be why Jane chooses to leave Rochester after discovering the existence of Bertha; it is in this moment that she again illustrates a self-sacrificing nature much like Helen’s. This not only creates interest because of the events triggered with in the novel from Helen’s teachings but Helen also gives a moral to the story. Miss Temple also has a semi-allegorical aspect; for eight years, she provides Jane with a place of refuge, which is mirrored in her name ‘Temple’. Miss Temple also provides Jane with a female role model she was lacking in early life. These two characters provide the novel with a religious undertone, and provide Bronte with a voice to comment upon her own religious beliefs and to question the morality with in Victorian society. Antoinette’s experience differs from Jane’s; the only character who is consistently kind to Antoinette throughout the novel is Christophine. In the case of Miss Temple and Helen Burns to Jane, Christophine becomes a role model to Antoinette. As a woman of colour, Christophine should be doubly disadvantaged by the patriarchal imperialism that rules the West Indies in the post-emancipation period. Yet, she is in many ways likened to the White men that are apparently more civilized than her. Christophine even seems to share the same values as these men: ‘Three children I have†¦each one a different father, but no husband, I thank my God’. However, whilst Jane finds an antidote to her passion and inner ‘id’, Christophine’s passion only adds fuel to Antoinette’s, resulting in her eventual destruction. Christophine’s character also adds much of the mystery to the novel, her practice of Obeah adds a sense of foreboding to the novel and paints her as a character to be feared, and compared to the ‘civilised’ Mr Rochester. Minor characters can also act as points of comparison for both the reader and the protagonist. In ‘Jane Eyre’ both Blanche Ingram and Bertha are provided as characters to be contrasted with Jane. Firstly Blanche Ingram is viewed by Jane as her love rival for Rochester’s attentions. In creating contrasting portraits of herself and Blanche, Jane emphasizes her own plainness. To Blanche, on the other hand, she gives the loveliest face she can imagine; here Janes passions become hyperbolic, as she cannot fully discipline her jealousy of Blanche. However Bronte’s use of words describes a much different character, she is described as having ‘raven-black’ hair arranged in glossy curls, and ‘brilliant black eyes’, which contrast with the ‘pure white’ clothes she wears. As with Janes descriptions of Mrs. Reed and her son John, ‘darkness’ often has negative connotations. Therefore, Janes (and so Bronte’s) description of Blanche, which emphasizes her dark, Spanish features, implies a negative side of her personality and creates dramatic irony i. e. It allows the reader to know that Blanche will not end up with Rochester instead of Jane. Within ‘Wide Sargasso Sea’ Bertha is most prominently compared with her mother, Bertha’s true name ‘Antoinette’ means ‘daughter of Annette’ and part of the reason why Rochester changes Antoinette’s name is that he fears she will become mad just like her. Unfortunately this is the fate that Antoinette meets; and not only does she suffer the ‘anglicising’ that her mother experienced at the hands of her husband but also the dissent in to madness. Providing her mother as a point of comparison not only foreshadows Antoinette’s fate, creating more suspense for the reader, but also causes the reader to question whether Antoinette’s madness was hereditary or a result of Rochester’s cruelty. In conclusion minor characters with in ‘Jane Eyre’ do create much of the interest It is a combination of their influence on our protagonist and the opportunity they provide for the authors to comment upon society and the protagonist they have created that makes minor characters such a point of interest for the reader.